Utilization of Partially Purified Milk Clotting Protease from Ginger Rhizome in the Manufacturing of Fresh Cheese

Authors

  • Bunty Maskey Central Department of Food Technology, Tribhuvan University, Dharan, Nepal
  • Trilochan Pandey Central Department of Food Technology, Tribhuvan University, Dharan, Nepal
  • Shiva Kumar Shrestha Central Department of Food Technology, Tribhuvan University, Dharan, Nepal
  • Nabindra Kumar Shrestha Central Department of Food Technology, Tribhuvan University, Dharan, Nepal
  • Megha Shrestha Central Department of Food Technology, Tribhuvan University, Dharan, Nepal
  • Dhan Bahadur Karki Central Department of Food Technology, Tribhuvan University, Dharan, Nepal

DOI:

https://doi.org/10.3126/hijost.v7i1.61159

Keywords:

Zingibain, Partial purification, RSM, Physicochemical properties, Sensory quality

Abstract

This study was carried out to utilize the partially purified ginger rhizome protease in fresh cheesemaking. The crude ginger rhizome extract was partially purified by employing 30-80% (NH4)2SO4 saturations (w/v). The maximum milk clotting activity was achieved at 50% saturation, exhibiting the purification fold of 1.64 and activity recovery of 85.76%. The milk pH and temperature for optimum time of coagulation and milk clotting activity were optimized by using response surface methodology (RSM). A numerical optimization study revealed that the optimum milk pH and temperature for producing cheese were 6.5 and 50 C respectively. The cheese produced by ginger protease had significantly (p<0.05) higher moisture, ash and yield, but lower fat content than the rennet cheese. No significant difference (p>0.05) was found in the calcium and protein content of both cheeses. The prepared cheese using ginger protease had significantly (p<0.05) higher spreadability, but lower aftertaste than the rennet cheese. However, non-significant difference (p>0.05) was observed in the flavor and overall acceptance between the two cheeses. Hence, this study demonstrated that the ginger rhizome protease has the potential to be utilized as an effective milk coagulating enzyme in the manufacturing of fresh cheese.

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Published

2023-12-31

How to Cite

Maskey, B., Pandey, T., Shrestha, S. K., Shrestha, N. K., Shrestha, M., & Karki, D. B. (2023). Utilization of Partially Purified Milk Clotting Protease from Ginger Rhizome in the Manufacturing of Fresh Cheese. Himalayan Journal of Science and Technology, 7(1), 4–17. https://doi.org/10.3126/hijost.v7i1.61159

Issue

Section

Original Research Articles