GC-MS PROFILING, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF NARDOSTACHYS JATAMANSI (D. DON) DC. RHIZOMES COLLECTED FROM TAPLEJUNG, GORKHA AND JUMLA DISTRICTS OF NEPAL
DOI:
https://doi.org/10.3126/jist.v31i1.87558Keywords:
Antimicrobial, Antioxidant, GC-MS, Nardostachys jatamansiAbstract
Nardostachys jatamansi (D. Don) DC. is a highly traded and critically endangered medicinal plant distributed in the high altitudes of Nepal. This study aims to investigate the chemical profile of essential oil from N. jatamansi collected from three different geographical regions of Nepal; Taplejung (NT), Gorkha (NG) and Jumla (NJ) and evaluate their antimicrobial and antioxidant activities. Greater amount of essential oil was obtained with NT and lesser amount was obtained with NJ. GC-MS analysis of NT revealed the identification of total of 35 compounds. In NG and NJ, 28 compounds in each were identified. Altogether 66 compounds were identified in NT, NG and NJ oil samples. Sesquiterpenes were the major compounds present in all three samples. Seven compounds like calarene, seychellene, α-patchoulene, valencene, 7-epi-α-selinene and patchouli alcohol were common in all three samples. Valeranone (37.42%), cadin-4-en-10-ol (28.07%), and patchouli alcohol (6.09%) were identified as the major components in NT. Similarly, valeranone (40.24%), cadin-4-en-10-ol (29.99%), patchouli alcohol (6.71%) with different composition were the major components in NG. In contrast, dihydrotagetone (21.30%), valencene (16.58%), β-pinene (12.56%), guaia-6,9-diene (10.93%) were the major components in NJ. In antimicrobial assay, only NJ showed activity with inhibition zone of 19.0 mm against Bacillus subtilis, Escherichia coli and Candida albicans. NT and NG oils were inactive. Because of the variation in chemical constituents, the oil samples showed different antibacterial activities. In antioxidant assay (DPPH), all the oil samples showed moderate activity.
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