Antimicrobial evaluation of polyaniline-coated Argeli paper for sustainable packaging
DOI:
https://doi.org/10.3126/njes.v13i2.85039Keywords:
Antibacterial, conjugated polymer, traditional Nepali paper, PANI, zone of inhibitionAbstract
Polyaniline (PANI)-coated traditional Nepali paper composites were synthesized via an in situ chemical oxidative polymerization process. Fourier Transform Infra-red Spectroscopy (FTIR), X-ray Diffraction (XRD), Optical Microscopy (OM) and Scanning Electron Microscopy (SEM) confirmed the PANI-coating. Additionally, the synthesized PANI powder (emeraldine salt) exhibited good antibacterial activity against Bacillus subtilis and Escherichia coli (zone of inhibition, ZOI: 0.5 cm each) and antifungal activity against Candida albicans (ZOI: 0.4 cm). In contrast, PANI-coated NK exhibited lower antimicrobial activity compared to powdered PANI. It was more effective against E. coli (ZOI: 0.3 cm) than against B. subtilis (ZOI: 0.2 cm). The antifungal activity against C. albicans was the lowest, with a ZOI of 0.1 cm. These findings demonstrate the potential of the composites for sustainable packaging applications with modest antimicrobial properties. Optimizing the polymerization process and material formulation could enhance their efficacy for eco-friendly active packaging systems.
Downloads
References
Adhikari, R., Bhandari, N.L., Causin, V., Le, H.H., Radusch, H., Michler, G.H., & Saiter, J.M. (2012). Study of morphology, mechanical properties, and thermal behavior of green aliphatic–aromatic copolyester/bamboo flour composites. Polymer Engineering & Science, 52(11), 2296–2303. https://doi.org/0.1002/pen.23335.
Aizamddin, M.F., Mahat, M.M., Ariffin, Z.Z., Nawawi, M.A., Jani, N.A., Amdan, N.A.N., & Sadasivuni, K.K. (2022). Antibacterial performance of protonated polyaniline-integrated polyester fabrics. Polymers, 14(13). https://doi.org/10.3390/polym14132617.
Ao, X., Liu, X., Dai, Z., & Zhu, A. (2025). The Preparation of polyaniline/graphene/polypropylene nanocomposite and its novel antibacterial activity. Polymer Composites, 46(3), 2794–2802. https://doi.org/10.1002/pc.29140.
Aryal, G.M., Kandel, K.P., Bhattarai, R.K., Giri, B., Adhikari, M., Ware, A., Han, S., George, G., Luo, Z., Gautam, B.R., & Neupane, B.B. (2022). Material properties of traditional handmade paper samples fabricated from cellulosic fiber of Lokta bushes. ACS Omega, 7(36), 32717–32726. https://doi.org/10.1021/acsomega.2c04398.
Brandelli, A. (2024). Nanocomposites and their application in antimicrobial packaging. Frontiers in Chemistry, 12, 1–13. https://doi.org/10.3389/fchem.2024.1356304.
Deng, Y., Tang, L., Zeng, G., Dong, H., Yan, M., Wang, J., Hu, W., Wang, J., Zhou, Y., & Tang, J. (2016). Enhanced visible light photocatalytic performance of polyaniline modified mesoporous single crystal TiO2 microsphere. Applied Surface Science, 387, 882–893. https://doi.org/10.1016/j.apsusc.2016.07.026.
Deuba, Y. (2015). Export barriers to internationalization: An investigation on Nepalese handmade paper industries. University of Nordland.
Duda-Chodak, A., Tarko, T., & Petka-Poniatowska, K. (2023). Antimicrobial compounds in food packaging. International Journal of Molecular Sciences, 24(3). https://doi.org/10.3390/ijms24032457.
Gautam, P., Groβmann, L., Basyal, O.P., Pradhan, S., Bhandari, N.L., Henning, S., Nase, M., & Adhikari, R. (2024). Argeli bast fiber as wonder reinforcing agent for biodegradable polymer composites. Nepal Journal of Environmental Science, 12(2), 1–8. https://doi.org/10.3126/njes.v12i2.68409.
Gautam, P., Groβmann, L., Pradhan, S., Bhandari, N.L., Nase, M., & Adhikari, R. (2024). Physicochemical and structural investigation of Argeli (Edgeworthia gardneri) bast fibers. Journal of Research Updates in Polymer Science, 13, 54–65. https://doi.org/10.6000/1929-5995.2024.13.07.
Giri, J., Adhikari, R., & Sapkota, J. (2024). Comparative study of polymer composites with cellulose microfibers from different plant resources. Advances in Polymer Technology, 2024, 1–11. https://doi.org/10.1155/2024/2396318.
Gizdavic-Nikolaidis, M.R., Bennett, J., Zujovic, Z., Swift, S., & Bowmaker, G.A. (2012). Characterization and antimicrobial efficacy of acetone extracted aniline oligomers. Synthetic Metals, 162(13–14), 1114–1119. https://doi.org/10.1016/j.synthmet.2012.04.031.
Green, J.B.D., Fulghum, T., & Nordhaus, M.A. (2011). A review of immobilized antimicrobial agents and methods for testing. Biointerphases, 6(4), MR13–MR28. https://doi.org/10.1116/1.3645195.
Hajlaoui, O., Khiari, R., Ajili, L., Batis, N., & Bergaoui, L. (2020). Design and characterization of Type I cellulose-polyaniline composites from various cellulose sources: A comparative study. Chemistry Africa, 3(3), 783–792. https://doi.org/10.1007/s42250-020-00148-1.
Hasanin, M.S., El Saied, H., Morsy, F.A., & Hassan Abdel Latif Rokbaa, H. (2023). Green nanocoating-based polysaccharides decorated with ZnONPs doped Egyptian Kaolinite for antimicrobial coating paper. Scientific Reports, 13(1), 1–14. https://doi.org/10.1038/s41598-023-38467-1.
Iconaru, S.L., Predoi, M.V., Chapon, P., Gaiaschi, S., Rokosz, K., Raaen, S., Motelica-Heino, M., & Predoi, D. (2021). Investigation of spin coating cerium-doped hydroxyapatite thin films with antifungal properties. Coatings, 11(4). https://doi.org/10.3390/coatings11040464.
Jose, A., Gizdavic-Nikolaidis, M., & Swift, S. (2023). Antimicrobial coatings: Reviewing options for healthcare applications. Applied Microbiology, 3(1), 145–174 https://doi.org/10.3390/applmicrobiol3010012.
Kalina, S., Kapilan, R., Wickramasinghe, I., & Navaratne, S.B. (2024). Potential use of plant leaves and sheath as food packaging materials in tackling plastic pollution: A review. Ceylon Journal of Science, 53(1), 21–37. https://doi.org/10.4038/cjs.v53i1.8145.
K.C., B.M., Lamichhane, J., Khanal, S.N., & Gauchan, D.P. (2024). Traditional utilization of bamboo in the Central Siwalik Region, Nepal. PLoS ONE, 19(1). https://doi.org/10.1371/journal.pone.0296886.
Ke, S., Ouyang, T., Zhang, K., Nong, Y., Mo, Y., Mo, Q., Wei, Y., & Cheng, F. (2019). Highly conductive cellulose network/polyaniline composites prepared by wood fractionation and in situ polymerization of aniline. Macromolecular Materials and Engineering, 304(7), 1900112. https://doi.org/10.1002/mame.201900112.
Kwon, S., Lee, W., Choi, J. W., Bumbudsanpharoke, N., & Ko, S. (2021). A facile green fabrication and characterization of cellulose-silver nanoparticle composite sheets for an antimicrobial food packaging. Frontiers in Nutrition, 8, 1–8. https://doi.org/10.3389/fnut.2021.778310.
Lai, T.T., Pham, T.T.H., van Lingen, M., Desaulniers, G., Njamen, G., Tolnai, B., Jabrane, T., Moineau, S., & Barnabé, S. (2022). Development of antimicrobial paper coatings containing bacteriophages and silver nanoparticles for control of foodborne pathogens. Viruses, 14(11). https://doi.org/10.3390/v14112478.
Lee, B.H., Kim, H.J., & Yang, H.S. (2012). Polymerization of aniline on bacterial cellulose and characterization of bacterial cellulose/polyaniline nanocomposite films. Current Applied Physics, 12(1), 75–80. https://doi.org/10.1016/j.cap.2011.04.045.
Manjunatha, S., Machappa, T., Ravikiran, Y.T., Chethan, B., & Sunilkumar, A. (2019). Polyaniline based stable humidity sensor operable at room temperature. Physica B: Condensed Matter, 561, 170–178. https://doi.org/10.1016/j.physb.2019.02.063.
Maráková, N., Humpolíček, P., Kašpárková, V., Capáková, Z., Martinková, L., Bober, P., Trchová, M., & Stejskal, J. (2017). Antimicrobial activity and cytotoxicity of cotton fabric coated with conducting polymers, polyaniline or polypyrrole, and with deposited silver nanoparticles. Applied Surface Science, 396, 169–176. https://doi.org/10.1016/j.apsusc.2016.11.024.
Maruthapandi, M., Saravanan, A., Gupta, A., Luong, J.H.T., & Gedanken, A. (2022). Antimicrobial activities of conducting polymers and their composites. Macromol, 2(1), 78–99. https://doi.org/10.3390/macromol2010005.
Poletto, M., Ornaghi Júnior, H.L., & Zattera, A.J. (2014). Native cellulose: structure, characterization and thermal properties. Materials, 7(9), 6105–6119. https://doi.org/10.3390/ma7096105.
Ramos, A.R., Tapia, A.K.G., Piñol, C.M.N., Lantican, N.B., del Mundo, M.L.F., Manalo, R.D., & Herrera, M.U. (2019). Morphological, electrical and antimicrobial properties of polyaniline-coated paper prepared via a two-pot layer-by-layer technique. Materials Chemistry and Physics, 238, 121972. https://doi.org/10.1016/j.matchemphys.2019.121972.
Razak, S.I.A., Sharif, N.F.A., & Nayan, N.H.M. (2014). Electrically conductive paper of polyaniline modified pineapple leaf fiber. Fibers and Polymers, 15(6), 1107–1111. https://doi.org/10.1007/s12221-014-1107-x.
Rehim, A.M.H., Yassin, M.A., Zahran, H., Kamel, S., Moharam, M.E., & Turky, G. (2020). Rational design of active packaging films based on polyaniline-coated polymethyl methacrylate/nanocellulose composites. Polymer Bulletin, 77(5), 2485–2499. https://doi.org/10.1007/s00289-019-02866-0.
Robertson, J., Gizdavic-Nikolaidis, M., Nieuwoudt, M. K., & Swift, S. (2018). The antimicrobial action of polyaniline involves production of oxidative stress while functionalisation of polyaniline introduces additional mechanisms. PeerJ, 2018(6), 1–36. https://doi.org/10.7717/peerj.5135.
Shalini, A., Nishanthi, R., Palani, P., & Jaisankar, V. (2016). One pot synthesis, characterization of polyaniline and cellulose/polyaniline nanocomposites: application towards in vitro measurements of antibacterial activity. Materials Today: Proceedings, 3(6), 1633–1642. https://doi.org/10.1016/j.matpr.2016.04.053.
Shi, N., Guo, X., Jing, H., Gong, J., Sun, C., & Yang, K. (2006). Antibacterial effect of the conducting polyaniline. Journal of Materials Science & Technology, 22(3), 289-290.
Sung, S.Y., Sin, L.T., Tee, T.T., Bee, S.T., Rahmat, A.R., Rahman, W.A.W.A., Tan, A.C., & Vikhraman, M. (2013). Antimicrobial agents for food packaging applications. Trends in Food Science and Technology, 33(2), 110–123. https://doi.org/10.1016/j.tifs.2013.08.001
Tang, S.J., Wang, A.T., Lin, S.Y., Huang, K.Y., Yang, C.C., Yeh, J.M., & Chiu, K.C. (2011). Polymerization of aniline under various concentrations of APS and HCl. Polymer Journal, 43(8), 667–675. https://doi.org/10.1038/pj.2011.43.
Tanpichai, S., Witayakran, S., Srimarut, Y., Woraprayote, W., & Malila, Y. (2019). Porosity, density and mechanical properties of the paper of steam exploded bamboo microfibers controlled by nanofibrillated cellulose. Journal of Materials Research and Technology, 8(4), 3612–3622. https://doi.org/10.1016/j.jmrt.2019.05.024.
Tirpude, R., & Singh, S. (2025). A comprehensive study of use of plant leaves in product packaging. Journal of Packaging Technology and Research, 9(2), 77–86. https://doi.org/10.1007/s41783-025-00184-7.
Turkten, N., Karatas, Y., Uyguner-Demirel, C.S., & Bekbolet, M. (2023). Preparation of PANI modified TiO2 and characterization under pre- and post- photocatalytic conditions. Environmental Science and Pollution Research, 30(51), 111182–111207. https://doi.org/10.1007/s11356-023-30090-x.
Vermeiren, L., Devlieghere, F., & Debevere, J. (2002). Effectiveness of some recent antimicrobial packaging concepts. Food Additives and Contaminants, 19, 163–171. https://doi.org/10.1080/02652030110104852.
Wasu, M.B., & Raut, A.R. (2014). Synthesis and characterization of polyaniline based conducting polymers. Journal of Chemistry and Chemical Sciences, 5(1), 70–117.
Yang, C., Chen, C., Pan, Y., Li, S., Wang, F., Li, J., Li, N., Li, X., Zhang, Y., & Li, D. (2015). Flexible highly specific capacitance aerogel electrodes based on cellulose nanofibers, carbon nanotubes and polyaniline. Electrochimica Acta, 182, 264–271. https://doi.org/10.1016/j.electacta.2015.09.096.
Youssef, A.M., El-Samahy, M.A., & Abdel Rehim, M.H. (2012). Preparation of conductive paper composites based on natural cellulosic fibers for packaging applications. Carbohydrate Polymers, 89(4), 1027–1032. https://doi.org/10.1016/j.carbpol.2012.03.044.
Zhang, F., Pang, Z., Dong, C., & Liu, Z. (2015). Preparing cationic cotton linter cellulose with high substitution degree by ultrasonic treatment. Carbohydrate Polymers, 132, 214–220. https://doi.org/10.1016/j.carbpol.2015.06.055.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Central Department of Environmental Science, Tribhuvan University

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.