Studies on Glycosaminoglycans Isolated from Bivalves Molluscs Tridacna maxima and Perna viridis

Authors

  • M. Arumugam Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai-608 502
  • T. Balasubramanian Department of Chemistry, Department of Chemical and Biochemical Engineering, Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa
  • M. Warda Department of Chemistry, Department of Chemical and Biochemical Engineering, Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa
  • R.J. Linhardt Department of Chemistry, Department of Chemical and Biochemical Engineering, Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa

DOI:

https://doi.org/10.3126/on.v7i1.2548

Keywords:

Anticoagulant, Bivalves molluscs, Glycosaminoglycans, Heparin, Invertebrates

Abstract

The glycosaminoglycans (GAGs) in two marine invertebrate molluscs such as Tridacna maxima and Perna viridis were analyzed. Both the species was found to contain variable amounts of GAGs in the form of heparin biomolecules as identified by metachromatic activity and agarose gel electrophoresis analysis. Anticoagulant property of the biomolecules was assessed by anti factor Xa activity. Their molecular weight was estimated as 15000 and 9000 Daltons through GPC-HPLC. The 1HNMR analysis of heparin was used to predict binding sites of the heparin. Structural characterization studies clearly demonstrated that heparin is the major GAGs constituents in the test animals.

Key words: Anticoagulant, Bivalves molluscs, Glycosaminoglycans, Heparin, Invertebrates

DOI: 10.3126/on.v7i1.2548

Our Nature (2009) 7:10-17

 

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Published

2010-01-25

How to Cite

Arumugam, M., Balasubramanian, T., Warda, M., & Linhardt, R. (2010). Studies on Glycosaminoglycans Isolated from Bivalves Molluscs Tridacna maxima and Perna viridis. Our Nature, 7(1), 10–17. https://doi.org/10.3126/on.v7i1.2548

Issue

Vol. 7 No. 1 (2009)

Section

Articles