Genetically Modified Organism (GMO) and Its Detection Method

Authors

  • Gyan Sundar Sahukhal Research Laboratory for Biochemistry and Biotechnology (RLABB), Universal Science College, Kathmandu, Nepal.
  • Bina Laxmi Jayana Nepal Academy of Science and Technology, Khumaltar, Lalitpur, Nepal
  • Nirmal Dhungana Nepal Academy of Science and Technology, Khumaltar, Lalitpur, Nepal
  • Nawa Raj Dahal Department of Food Technology and Quality Control, Babarmahal, Nepal
  • Ganesh Dawadi Department of Food Technology and Quality Control, Babarmahal, Nepal
  • Shova Shrestha Tri-Chandra Multiple College, Department of Microbiology. Ghantaghar, Nepal.
  • Vishwanath Prasad Agrawal Research Laboratory for Biochemistry and Biotechnology

Keywords:

GMO, Beneficial Aspects, Harmful Aspects, Detection Strategy

Abstract

Genetically modified (GM) crops currently account for 29% of crop production worldwide. Despite a lack of regulations to provide for food labelling that allows for consumer preference, many products carry negative or positive labels with regard to genetic modification. Regulatory demands of labelling and traceability of GMOs in the food chain need suitable sampling protocols and analytical methods. At present, two main techniques, the DNA-based polymerase chain reaction (PCR) and the protein-based enzyme-linked immunosorbent assay (ELISA) are most widely used, although new technologies, such as microarrays, have been also developed. The main objective of this review paper is to assess the detection methods both protein based and PCR based as described by different researchers, which represents the state-of-the-art technique for GMO detection in food.

Key words: GMO; Beneficial Aspects; Harmful Aspects; Detection Strategy

Journal of Food Science & Technology Nepal Vol 4, September, 2008, Page: 18-22

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Abstract
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Author Biographies

Gyan Sundar Sahukhal, Research Laboratory for Biochemistry and Biotechnology (RLABB), Universal Science College, Kathmandu, Nepal.

Professor; Research Laboratory for Biochemistry and Biotechnology (RLABB), Universal Science College, Kathmandu, Nepal.

Bina Laxmi Jayana, Nepal Academy of Science and Technology, Khumaltar, Lalitpur, Nepal

Professor; Nepal Academy of Science and Technology, Khumaltar, Lalitpur, Nepal

Nirmal Dhungana, Nepal Academy of Science and Technology, Khumaltar, Lalitpur, Nepal

Professor; Nepal Academy of Science and Technology, Khumaltar, Lalitpur, Nepal

Nawa Raj Dahal, Department of Food Technology and Quality Control, Babarmahal, Nepal

Section Officer; Department of Food Technology and Quality Control, Babarmahal, Nepal

Ganesh Dawadi, Department of Food Technology and Quality Control, Babarmahal, Nepal

Section Officer; Department of Food Technology and Quality Control, Babarmahal, Nepal

Shova Shrestha, Tri-Chandra Multiple College, Department of Microbiology. Ghantaghar, Nepal.

Professor; Tri-Chandra Multiple College, Department of Microbiology. Ghantaghar, Nepal.

Vishwanath Prasad Agrawal, Research Laboratory for Biochemistry and Biotechnology

Professor; Research Laboratory for Biochemistry and Biotechnology (RLABB), Universal Science College, Kathmandu, Nepal.

Published

2009-06-30

How to Cite

Sahukhal, G. S., Jayana, B. L., Dhungana, N., Dahal, N. R., Dawadi, G., Shrestha, S., & Agrawal, V. P. (2009). Genetically Modified Organism (GMO) and Its Detection Method. Journal of Food Science and Technology Nepal, 4, 18–22. Retrieved from https://nepjol.info/index.php/JFSTN/article/view/1989

Issue

Vol. 4 (2008)

Section

Articles

License

The author will be  the copyright holder of this open access journal - 'Journal of Food Science and Technology Nepal (JFSTN)'.