The Capacity of Lignin to Enhance the Phthorimaea opercullela Granulovirus (PhopGV) Stability in Laboratory Condition

Authors

  • Shiva Shankar Bhattarai Institute of Agriculture and Animal Sciences, Tribhuvan University
  • Sunil Aryal Entomology Division, Nepal Agriculture Research Council, Khumaltar
  • Resham Bahadur Thapa Department of Entomology, Tribhuvan University, Institute of Agriculture and Animal Sciences, Kirtipur

DOI:

https://doi.org/10.3126/ijasbt.v3i2.12481

Keywords:

Raw PhopGV, lab persistence, lignin, stability

Abstract

The lab persistence of Phthorimaea opercullela granulovirus (PhopGV) with and without lignin for egg bioassay with different five virus concentrations (lab experiment- 60, 10, 1.67, 0.28 and 0.046 LE/l 20- 0.015432 LE/l ) to determine the inactivation curve under artificial UVlight, assess the effect and verify the protective capacity of lignin with PhopGV for virus preparations suspensions of raw PhopGV alone and mixed with lignin (1%) used as UV protectants was conducted in simply completely randomised design in the laboratory condition. The LC50 value for PhopGV in lab condition was 1x10-3 LE/l with the slope of the probit regression line 0.51. Similarly, half inactivation speed with lignin was 4.58 days compared to 0.559 days without lignin in lab condition. Inactivation speed was decreased by 87.8 % in case of lignin applied in lab condition. So, the suppression of PTM with virus combined with lignin gave cost effective measures through the use of low dosages. In lab experiment, the control mortality of 19.6 % was probable for all the controls of lignin present and absent. This revealed that there was no effect of lignin alone on target insects’ survival.

Int J Appl Sci Biotechnol, Vol 3(2): 256-260

DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12481

 

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Author Biography

Shiva Shankar Bhattarai, Institute of Agriculture and Animal Sciences, Tribhuvan University

Assistant Professor, Department of Horticulture and Plant Protection

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Published

2015-06-25

How to Cite

Bhattarai, S. S., Aryal, S., & Thapa, R. B. (2015). The Capacity of Lignin to Enhance the Phthorimaea opercullela Granulovirus (PhopGV) Stability in Laboratory Condition. International Journal of Applied Sciences and Biotechnology, 3(2), 256–260. https://doi.org/10.3126/ijasbt.v3i2.12481

Issue

Section

Research Articles: Biological Sciences