International Journal of Applied Sciences and Biotechnology https://nepjol.info/index.php/IJASBT <p>An International online journal in the English language published quarterly (March. June, September and December) by SEM-Biotech Publishing. Full text articles available. Content also available on the journal's own <a title="IJASBT" href="http://www.ijasbt.org/" target="_blank" rel="noopener">website</a>.</p> en-US editor.ijasbt@gmail.com (Dr Umesh P. Shrivastava) sioux.cumming@ubiquitypress.com (Sioux Cumming) Fri, 29 Dec 2023 13:56:52 +0000 OJS 3.3.0.6 http://blogs.law.harvard.edu/tech/rss 60 Unveiling The Potential of Jimbu (Allium przewalskianum): Bioactive Compounds, Antioxidant, and Antimicrobial Properties of a Native Himalayan Spice Herb https://nepjol.info/index.php/IJASBT/article/view/59326 <p>Jimbu (<em>Allium przewalskianum</em>), is an indigenous Himalayan spice herb in Nepal and has a long history of culinary use, yet its potential beyond home kitchens remains largely unexplored due to limited studies and exposure. It does not require extensive input of cash, machinery, land, or labor to profit from, making it an excellent and valuable source of pharmaceutical ingredients owing to its bioactive compounds. We investigated its antioxidant and antimicrobial properties. Quantitative analysis of Jimbu extracts revealed the presence of bioactive compounds, including Oleoresin (extracted using acetone and methanol), polyphenols, flavonoids, and tannins. The methanol extract of. <em>A</em>. przewalskianum exhibited the highest polyphenol content (8.11±0.24 mg GAE/g), flavonoid content (5.28±0.29 mg GAE/g) and tannin content (0.923±0.02 mg GAE/g). The antioxidant potential of Jimbu was seen to be increased with an increase in concentration, reaching 58.79% at 400µg/ml, with an IC50 value of 303.58µg/ml for DPPH radical scavenging activity. Likewise, the yield of oleoresin was obtained as (10.86±0.27) from acetone and (7.8±0.20) from methanol. Similarly, sensory evaluation through One-way ANOVA highlighted concentrations of 0.07% and 0.1% of Oleoresin to be most favored. Furthermore, sensory evaluation of the Jimbu extracts in minced chicken meat exhibited remarkable antimicrobial activity against Salmonella along with a significant reduction in the colony forming units (CFU). This study shows that the extracts of <em>A. przewalskianum</em> could be utilized as a potential medicinal plant for the development of effective drugs against pathogenic bacteria. Meanwhile, it provides opportunities for economic benefits and a new potential revenue source for rural communities in the Himalayan region of Nepal.</p> <p><em>Int. J. Appl. Sci. Biotechnol. Vol 11(4): 171-180.</em></p> Shreejana Pandey, Guheswari Chataut, Suja Maharjan, Dev Raj Maidali, Keshab Bhattarai Copyright (c) 2023 International Journal of Applied Sciences and Biotechnology http://creativecommons.org/licenses/by-nc/4.0 https://nepjol.info/index.php/IJASBT/article/view/59326 Fri, 29 Dec 2023 00:00:00 +0000 Study on Cultivation of Wheat by Temperature Control with Adequate Irrigation in the Fields of South India https://nepjol.info/index.php/IJASBT/article/view/59870 <p>This investigation was conducted during the Zaid season of 2023 to evaluate the performance of four wheat variants was conducted in the agricultural plots of Nindra Mandal, Chittoor District, Andhra Pradesh, India. (13.3831°N 79.6963°E). The experimental plots were laid out in open farming design with adjacent block design with for variants of wheat: Chhoti Lerma, Kalyan sona, Sharbati Sonora and Sonalika with control being GW173. Growth and yield were found to be good in open farming comprising the nutrient rich red soil with pH 5.6. Growth parameters viz. plant height, diameter and crown width were observed. Grain yield attributes like wheat germination count, leaf area index and plant height were found higher in Kalyan sona. Harvest Index and Nutrients in each variety have been recorded in which the grains of Kalyan sona and Sonalika showed to be compatible to be cultivated in summer.</p> <p><em>Int. J. Appl. Sci. Biotechnol. Vol 11(4): 181-185.</em></p> Jwala Priyadarshini P.S. Copyright (c) 2023 International Journal of Applied Sciences and Biotechnology http://creativecommons.org/licenses/by-nc/4.0 https://nepjol.info/index.php/IJASBT/article/view/59870 Fri, 29 Dec 2023 00:00:00 +0000 Antimicrobial Activity and Phytochemical Screening of Traditional Medicinal Plants https://nepjol.info/index.php/IJASBT/article/view/61161 <p>In Nepal, traditional medicinal plants and herbs have long been relied upon for treating various diseases. This study was aimed to explore the antimicrobial properties of commonly used traditional medicinal plants. This study was conducted from December 2022 to June 2023 at the Department of Microbiology, St. Xavier's College, Maitighar, Kathmandu. In this study, antimicrobial property of 12 plants were tested against four ATCC cultures of bacteria- <em>Staphylococcus aureus </em>(ATCC 6538P), <em>Staphylococcus epidermidis </em>(ATCC 12228), <em>Escherichia coli </em>(ATCC 8739) and <em>Pseudomonas</em> <em>aeruginosa</em> (ATCC 9027). The extracts were prepared using Soxhlet's apparatus with ethanol and distilled water as solvents. The ethanolic extract of <em>S</em>. <em>aromaticum </em>had the highest percentage yield The ethanolic extract of <em>O</em>. <em>sanctum </em>showed highest potential against <em>S</em>. <em>aureus </em>with a zone of inhibition of 21mm. The ethanolic extract of <em>S</em>. <em>aromaticum </em>demonstrated antimicrobial activity against <em>S</em>. <em>aureus</em>, <em>E</em>. <em>coli</em>, <em>P</em>. <em>aeruginosa</em>, and <em>S</em>. <em>epidermidis</em>, with respective zones of inhibition of 18mm, 16mm, 18mm, and 20mm. The distilled water extract of <em>A. marmelos </em>displayed the highest antimicrobial activity against <em>E. coli, </em>with a zone of inhibition of 18mm. Phytochemical screening revealed that both extracts of S. <em>aromaticum </em>had the highest presence of phytochemicals. The ethanol extract of <em>Z</em>. <em>armatum </em>exhibited the highest antibacterial activity, with a minimum inhibitory concentration (MIC) value of 2mg/ml against <em>S</em>. <em>aureus</em>. The study of the selected plants extracts confirmed their efficacy as natural antimicrobials against the tested organisms, suggesting their potential for novel drug development in treating infectious diseases.</p> <p><em>Int. J. Appl. Sci. Biotechnol. Vol 11(4): 186-196.</em></p> Nikita Mahat, Niharika Bhattarai, Milan Thapa, Mousam Lawati, Anup Basnet Copyright (c) 2023 International Journal of Applied Sciences and Biotechnology http://creativecommons.org/licenses/by-nc/4.0 https://nepjol.info/index.php/IJASBT/article/view/61161 Fri, 29 Dec 2023 00:00:00 +0000 A Comprehensive Value Chain Analysis of Mango Production and Distribution in Saptari District, Nepal https://nepjol.info/index.php/IJASBT/article/view/60370 <p>Mango is an important fruit crop grown widely in the Saptari district of Nepal. A study was conducted in March and June 2022 under PMAMP-PIU (Prime Minister Agriculture Modernization Project-Project Implementation Unit), Saptari (PMAMP-PIU, 2074/75) to analyze the value chain of Mango in Saptari, which explored the functional and economic linkage among the actors. A total of 60 producers, 10 traders, 5 retailers and 2 key informants were interviewed. Data regarding production, post-harvest handling and marketing were analyzed by using descriptive and analytical methods with SPSS (version 26) and MS Excel 2021. Qualitative and quantitative analysis was undertaken in this study to generate insights into the limitations and opportunities of the mango value chain which can be used to establish the critical control points. The study identified key actors of Mango VC as input suppliers, producers, contract farmers, village-level collectors, wholesalers, retailers and consumers. Likewise, 4 marketing channels were identified in the study area. Economic analysis shows the cost of production per hectare was $1,453.71. Benefit cost analysis showed mango sub-sector is profitable business with BC ratio of 2.02. The average return was $2942.88/ha. The producers’ share was 55.28% in the proximal market and 32.25% in the distant market. Infestation of disease, insect and pest and lack of processing and post-harvest technology was major production problem.</p> <p><em>Int. J. Appl. Sci. Biotechnol. Vol 11(4): 197-208.</em></p> Puja Teyung, Gaurab Luitel Copyright (c) 2023 International Journal of Applied Sciences and Biotechnology http://creativecommons.org/licenses/by-nc/4.0 https://nepjol.info/index.php/IJASBT/article/view/60370 Fri, 29 Dec 2023 00:00:00 +0000 Correlation Between Teeth Alignment and Oral Diseases in New India https://nepjol.info/index.php/IJASBT/article/view/61163 <p>Systemic Health goes together with oral health. In India, Oral health remains a big challenge. It is connected tightly to overall individual’s health. Basic oral hygiene such as brushing teeth twice a day is still foreign to most, while such practices are widely practiced in the west. In India, economic factors, coupled with normally accepted behavior and acceptance of poor oral hygiene, prevention of awareness contributed to vast populace with dental issues, like cavities, gum diseases, premature teeth loss, and oral cancers in some cases. With almost 60% of 1.4 billion living in rural areas, initiatives like oral hygiene awareness, preventative care, lifestyle changes and increased dental facilities including universities, colleges and dental care facilities are needed today.</p> <p>We investigate whether there is an association between awareness of teeth alignment, good oral health to overall systemic health. We developed a questionnaire for dental patients falling between the age of 13 - 21 years. This range was selected as most of the patients are old enough to know their oral habits, and other health issues. The patient’s data was collected over a range of 8 months (April 2023 to November 2023) between two states in southern India. The research had 191 patients who were from rural areas, and their awareness on oral hygiene activities were found to be far worse than their urban counterparts (both genders). Based on the data collected, we establish a close relationship between awareness of teeth alignment, to gum bleeding, caries, and pollinosis in young Indians.</p> <p><em>Int. J. Appl. Sci. Biotechnol. Vol 11(4): 209-216.</em></p> Deepak T.A. , Avinash Tejasvi M.L. Copyright (c) 2023 International Journal of Applied Sciences and Biotechnology http://creativecommons.org/licenses/by-nc/4.0 https://nepjol.info/index.php/IJASBT/article/view/61163 Fri, 29 Dec 2023 00:00:00 +0000 Synthesis and Characterization of Azo-based Compounds as Energy Storage Materials https://nepjol.info/index.php/IJASBT/article/view/61164 <p>Two redox active azo-based materials have been synthesized and characterized by elemental analysis (C, H, N), IR, NMR (<sup>1</sup>H &amp; <sup>13</sup>C) and single crystal X-ray diffraction analysis. Both the azo-based materials, the ligand <strong>1</strong> and its MOF <strong>2</strong> exhibit good electrochemical performances but the capacity of MOF <strong>2</strong> is higher than the ligand 1.</p> <p><em>Int. J. Appl. Sci. Biotechnol. Vol 11(4): 217-221.</em></p> Pramod Kumar Yadav Copyright (c) 2023 International Journal of Applied Sciences and Biotechnology http://creativecommons.org/licenses/by-nc/4.0 https://nepjol.info/index.php/IJASBT/article/view/61164 Fri, 29 Dec 2023 00:00:00 +0000