Induction, Proliferation and Differentiation of Callus in Paris polyphylla Sm. through Leaf Culture

Authors

  • Chandra Bahadur Thapa Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal
  • Krishna Kumar Pant Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal
  • Hari Datta Bhattarai Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal
  • Mahendra Thapa Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal
  • Bijaya Pant Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal https://orcid.org/0000-0001-5614-6031

DOI:

https://doi.org/10.3126/jnba.v5i1.63741

Keywords:

2,4-D, Callus, GA3, Growth index, Primary culture

Abstract

Paris polyphylla Sm. is a vulnerable medicinal plant employed in the treatment of various ailments. This study seeks to establish a protocol for callus induction, proliferation, and differentiation of P. polyphylla. Immature leaf explants were cultured on MS medium with varying concentrations of plant growth regulators (PGRs), including 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kn), 6-benzylaminopurine (BAP), Thidiazuron (TDZ), α-Naphthalene acetic acid (NAA), and Gibberellic acid (GA3), along with 10% coconut water. After 12 weeks of primary culture, the optimal callus induction was observed in MS medium supplemented with 0.25 mg/l 2,4-D + 0.5 mg/l Kn. In the secondary culture at 8 weeks, the best callus proliferation, as determined by callus weight or growth index, occurred in MS medium supplemented with 2.0 mg/l BAP alone, 2.0 mg/l Kn alone, 1.0 mg/l TDZ alone, combinations of 2.0 mg/l Kn + 1.0 mg/l BAP + 2.0 mg/l GA3, and combinations of 0.5 mg/l NAA + 2.0 mg/l BAP + 2.0 mg/l GA3, as well as 10% coconut water. Furthermore, callus differentiation into mini rhizomes with root primordia was successfully achieved in MS media containing 2.5 mg/l Kn and 10% coconut water. This study reports, for the first time, the formation and differentiation of callus from leaf explants in P. polyphylla. Large-scale callus generation from leaf explants has the potential to enhance the production of bioactive secondary metabolites for therapeutic purposes and facilitate the development of plantlets through organogenesis.

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Published

2024-03-14

How to Cite

Thapa, C. B., Pant, K. K., Bhattarai, H. D., Thapa, M., & Pant, B. (2024). Induction, Proliferation and Differentiation of Callus in Paris polyphylla Sm. through Leaf Culture. Journal of Nepal Biotechnology Association, 5(1), 8–15. https://doi.org/10.3126/jnba.v5i1.63741

Issue

Section

Research Articles