Common Buckwheat-based EST Primers in the Genome of other Species of <i>Fagopyrum</i>

Authors

  • BK Joshi Nepal Agricultural Research Council, Kathmandu
  • K Okuno Laboratory of Plant Breeding, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • R Ohsawa Laboratory of Plant Breeding, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • T Hara Laboratory of Plant Breeding, Graduate School of Life and Environmental Sciences, University of Tsukuba

DOI:

https://doi.org/10.3126/narj.v7i0.1863

Keywords:

Common buckwheat, EST markers, Fagopyrum species, transferability

Abstract

If the EST primers designed for one species can be used in related species, then the cost involved
in developing markers for DNA fingerprinting, genetic relationship studies, mapping, etc. for
other species is significantly reduced. We tested the applicability of 17 EST primers developed
from common buckwheat in other wild and cultivated Fagopyrum species. A total of 18
accessions consisting of 4 subspecies and 2 species were used. Sequences of 93 cDNA clones
were used to design primers using Primer3. Amplification products were different in band
intensity. In most of the cases, the bands of F. homotropicum were with high intensity. All
primers showed single band except in Accession C9022. Three primers 23, 31 and 69 produced
very clear singe band. All primers amplified the genomic DNA of F. homotropicum (2x). Eight
primers amplified the DNA of all accessions. Results indicated that the transferability of EST
markers developed for common buckwheat decreased with an increase in genetic distance
between them.

Key words: Common buckwheat; EST markers; Fagopyrum species; transferability

DOI: 10.3126/narj.v7i0.1863

Nepal Agriculture Research Journal
Vol.7 2006 pp.27-36

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How to Cite

Joshi, B., Okuno, K., Ohsawa, R., & Hara, T. (2009). Common Buckwheat-based EST Primers in the Genome of other Species of <i>Fagopyrum</i>. Nepal Agriculture Research Journal, 7, 27–36. https://doi.org/10.3126/narj.v7i0.1863

Issue

Section

Plant Breeding, Genetics and Biotechnology