In Vitro Evaluation of the Anti-Bacterial Activity of Aloe Vera and Mint Extract against Bacterial Isolates from Facial Acne

Authors

  • Soma Kanta Baral Department of Laboratory Medicine, Manmohan Memorial Institute of Health Sciences, Kathmandu, Nepal https://orcid.org/0000-0001-7244-6501
  • Nabina Bhaila Department of Laboratory Medicine, Manmohan Memorial Institute of Health Sciences, Kathmandu, Nepal
  • Samita Bhasima Department of Laboratory Medicine, Manmohan Memorial Institute of Health Sciences, Kathmandu, Nepal https://orcid.org/0009-0007-5398-8867

DOI:

https://doi.org/10.3126/jmmihs.v11i1.94449

Keywords:

Acne, Aloe vera, Essential oil, MRSA, MSSA, MIC, MBC

Abstract

Introduction: Acne is a common dermatological condition often associated with bacterial colonization, primarily Staphylococcus aureus and Staphylococcus epidermidis. The increasing resistance to conventional antibiotics has prompted the exploration of plant-based alternatives. This study investigates the antibacterial effects of Aloe vera extract and Mentha spicata essential oil on acne-associated bacterial isolates.

Method: A cross-sectional, laboratory-based study was conducted involving 51 facial acne samples. Bacterial isolates were identified using standard biochemical tests. Mentha spicata essential oil was obtained via hydro-distillation, while Aloe vera extract was prepared using hot air oven drying followed by dissolution in Dimethyl sulfoxide. The antibacterial activity was assessed using the agar well diffusion method.

Result: Out of 51 acne samples, 48 (94.11%) showed bacterial growth. Staphylococcus epidermidis was the most common isolate, followed by Staphylococcus aureus including Methicillin-resistant (MRSA) and Methicillin-sensitive (MSSA) strains. Mentha spicata essential oil exhibited an antibacterial effect against 81.13% of isolates (MIC and MBC: 25 µL/mL), while Aloe vera extract was active against 58.5% (MIC and MBC: 50 µL/mL).

Conclusion: Both Aloe vera and Mentha spicata demonstrated inhibitory effects against acne-associated bacteria. The stronger antibacterial activity of mint essential oil suggests its greater therapeutic potential in acne management.

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Published

2026-06-05

How to Cite

Baral, S. K., Bhaila, N., & Bhasima, S. (2026). In Vitro Evaluation of the Anti-Bacterial Activity of Aloe Vera and Mint Extract against Bacterial Isolates from Facial Acne. Journal of Manmohan Memorial Institute of Health Sciences, 11(1), 51–57. https://doi.org/10.3126/jmmihs.v11i1.94449

Issue

Vol. 11 No. 1 (2026)

Section

Articles

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Copyright (c) 2026 Soma Kanta Baral, Nabina Bhaila, Samita Bhasima

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