Brief Survey of Antibiogram Profile of Commercially Available Probiotic Preparations in City Ranchi-India

Abstract

Probiotic microorganisms are frequently utilized in pharmaceutical and food formulations due to their beneficial effects on human health and gut microbiota. However, the unforeseen emergence of resistance to antibiotics among such probiotic microbes invites attention for the careful evaluation of the safety and therapeutic applicability of these probiotic microbes. The present study investigated the antibiogram profile of such isolates procured from locally available pharmaceutical probiotic formulations. Antibiotic susceptibility test revealed considerable variation among the isolates six. All six isolates exhibited complete sensitivity towards gentamicin and showed predominant sensitivity to amikacin, vancomycin, ampicillin-sulbactam, chloramphenicol, amoxiclav, and ampicillin. Intermediate resistance was recorded against ciprofloxacin, tetracycline, nalidixic acid, azithromycin, and kanamycin. Certain isolates revealed resistance to clindamycin, ceftazidime, erythromycin, and penicillin. The study highlights that probiotic strains possessing intrinsic/non-transferable resistance may provide advantages during concurrent antibiotic therapy by maintaining gut microbial balance. The occurrence of multidrug resistance, particularly on mobile genetic elements, among some isolates raises biosafety concerns regarding the possible dissemination of resistance. Besides such resistant microbes may, sometimes, compromise the efficacy of therapeutic antibiotics. Overall, this investigation emphasizes that antibiotic resistance profiling should be considered a critical criterion in the development of probiotic formulations. A comprehensive evaluation of resistance mechanisms, transmissibility, and biosafety parameters will ensure the safe, effective, and sustainable utilization of probiotics in food, pharmaceutical and clinical setups

Int. J. Appl. Sci. Biotechnol. Vol 14(2): 65-77.