Simple and Rapid Liquid Chromatography Method for Determination of Rifabutin in Plasma

Authors

  • AK Hemanth Kumar National Institute for Research in Tuberculosis, Chetput, Chennai,
  • V Sudha National Institute for Research in Tuberculosis, Chetput, Chennai,
  • Geetha Ramachandran National Institute for Research in Tuberculosis, Chetput, Chennai,

Keywords:

tuberculosis, rifabutin, liquid chromatography

Abstract

A high performance liquid chromatographic method for determination of rifabutin in human plasma was  developed. The method involved deproteinisation of the sample with acetonitrile and analysis of the  supernatant using a reversed-phase C18 column (250mm) and UV detection at a wavelength of 265nm.  The assay was specific for rifabutin and linear from 0.025 to 10.0μg/ml. The relative standard deviation  of intra- and inter-day assays was lower than 10%. The method was able to remove interfering materials  in plasma, yielding an average recovery of rifabutin from plasma of 101%. Due to its simplicity, the assay  can be used for pharmacokinetic studies of rifabutin.

SAARC Journal of Tuberculosis, Lung Diseases & HIV/AIDS; 2012; IX(2) 26-29

DOI: http://dx.doi.org/10.3126/saarctb.v9i2.7975

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Published

2013-04-23

How to Cite

Simple and Rapid Liquid Chromatography Method for Determination of Rifabutin in Plasma. (2013). SAARC Journal of Tuberculosis, Lung Diseases and HIV AIDS, 9(2), 26-29. https://doi.org/10.3126/saarctb.v9i2.7975

Issue

Vol. 9 No. 2 (2012)

Section

Articles

License

Copyright © SAARC Tuberculosis and HIV/AIDS Centre (STAC), all rights reserved, no part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means without prior permission of the STAC.

How to Cite

Simple and Rapid Liquid Chromatography Method for Determination of Rifabutin in Plasma. (2013). SAARC Journal of Tuberculosis, Lung Diseases and HIV AIDS, 9(2), 26-29. https://doi.org/10.3126/saarctb.v9i2.7975