A SIMPLE AND RAPID LIQUID CHROMATOGRAPHY METHOD FOR DETERMINATION OF LEVOFLOXACIN IN PLASMA

Authors

  • A. K. Hemanth Kumar Department of Biochemistry & Clinical Pharmacology, NIRT (ICMR) Chetput, Chennai
  • V. Sudha Department of Biochemistry & Clinical Pharmacology, NIRT (ICMR) Chetput, Chennai
  • G Ramachandran Department of Biochemistry & Clinical Pharmacology, NIRT (ICMR) Chetput, Chennai

DOI:

https://doi.org/10.3126/saarctb.v14i1.17726

Keywords:

Levofloxacin, Plasma, HPLC, Fluoroquinolnes

Abstract

Introduction: Levofloxacin (LFX) is one of the second line anti-tuberculosis drugs used in the treatment of multi drug resistant tuberculosis. Monitoring of LFX concentrations in plasma may be valuable to study its pharmacokinetics and drug-drug interaction when co-administered with other anti-tuberculosis drugs. We developed a high performance liquid chromatic method of determination of LFX in plasma.

Methodology: The method involved deproteinisation of the sample with perchloric acid and analysis of the supernatant using a reversed-phase C18 column (150mm) and fluorescence detection at an excitation wavelength of 290 nm and an emission wavelength of 460 nm.

Results: The assay was specific for LFX and linear from 0.25 to 10.0μg/ml. The relative standard deviation of intra- and inter-day assays was lower than 10%. The average recovery of LFX from plasma was 99%.

Conclusion: A sensitive, specific and validated method for quantitative determination of LFX in plasma was developed .Due to its simplicity; the assay can be used for pharmacokinetic studies of LFX.

SAARC J TUBER LUNG DIS HIV/AIDS, 2017; XIV(1), page: 27-32

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Published

2017-07-12

How to Cite

Kumar, A. K. H., Sudha, V., & Ramachandran, G. (2017). A SIMPLE AND RAPID LIQUID CHROMATOGRAPHY METHOD FOR DETERMINATION OF LEVOFLOXACIN IN PLASMA. SAARC Journal of Tuberculosis, Lung Diseases and HIV/AIDS, 14(1), 27–32. https://doi.org/10.3126/saarctb.v14i1.17726

Issue

Vol. 14 No. 1 (2017)

Section

Articles

License

Copyright © SAARC Tuberculosis and HIV/AIDS Centre (STAC), all rights reserved, no part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means without prior permission of the STAC.