Standardization of Storage Conditions and Duration on Picroside-I and Picroside-II in Raw Material of Drug Kutki (Picrorhiza kurroa Royle ex Benth.)

Abstract

The study of the proper storage of medicinal plants after being harvested becomes necessary to minimize the loss of active constituents from microbial reactions, heat, moisture, light and enzymes etc. In the present study, fresh rhizomes of Picrorhiza kurroa (Kutki) were collected from 2600 m to 3300 m altitude of Himachal Pradesh (Rohtang area), India. The harvested material was washed to remove soil and other adhering materials, and then cut into small pieces and dried under shade. The air dried samples were packed in the different packing materials (i.e. Gunny bags or poly bags), stored either at room temperature or in refrigerator at 4° to 6 °C or in humid environment (85% humidity and 25 °C) for different storage durations and then analyzed their chemical constituents bimonthly. The study showed that the content of picroside-I and picroside-II decreased with the increase in storage duration irrespective of storage condition. However, the loss in picroside-I and picroside-II with storage was maximum when “Kutki” samples were stored under humid condition (85% humidity, 25 °C) and minimum loss was observed when samples were stored at low temperature (4-6 °C). So, to minimize the loss of picroside-I and picroside-II content in the drug Kutki during storage, Kutki must be stored at low temperature.