Susceptibility Pattern of Gram Negative Urine Pathogens with Exclusion of Escherichia coli to Quinolone/ Fluoroquinolone Antibiotics

Abstract

Quinolone/Fluoroquinolones group of antibiotics are frequently used for the treatment of urinary tract infection (UTI). Because of high frequency of Gram negative bacterial infection in UTI and emerging resistant urinary pathogen in spite of lower prevalence with exclusion of Escherichia coli, the susceptibility pattern was subjected in this study. The study was conducted at National Public Health Laboratory (NPHL) Teku, Kathmandu with an objective to study the antibiotic Quinolone/Fluoroquinolones susceptibility pattern. Out of 550 urine samples, 100 (18.18%) bacteria were isolated from NPHL. Also, 69 isolates from Blue-Cross Hospital and 83 isolates from Medicare Hospital were collected. Altogether 252 urine isolates, showing significant growth in MacConkey Agar and blood agar were identified by standard microbiological techniques. Among 62 Gram negative isolates other than Escherichia coli subjected for the study; Proteus mirabilis (27.42%), Proteus vulgaris (11.29%), Klebsiella oxytoca (12.90%), Klebsiella pneumoniae (17.75%), Citrobacter freundii (8.06%), Enteribacter cloaecae (6.45%), Pseudomonas aeruginosa (12.90%), Acinetobacter spp. (3.23%). Altogether eight types of antibiotics belonging to quinolone/fluoroquinolone group were used in this study. Among them, norfloxacin was found most sensitive and nalidixic acid was most resistant against Gram negative isolates.

DOI: http://dx.doi.org/njst.v15i2.12119

Nepal Journal of Science and Technology Vol. 15, No.2 (2014) 77-84