Quantitation of α_1A and α_1D-adrenoceptor mRNA in prostate tissues from patients with symptomatic benign prostatic hyperplasia

Abstract

Background: To treat symptomatic benign prostatic hyperplasia α₁-adrenoceptor antagonists with little antagonism at α_1b-adrenoceptor were used to avoid orthostatic hypotension. In benign prostatic hyperplasia tissues α_1D-adrenoceptor are thought to predominate, but in the Japanese experience, either α_1A- or α_1D- adrenoceptor antagonists can alleviate benign prostatic hyperplasia symptoms. We hypothesized that prostatic expression of α_1A- and α_1D-adrenoceptor varies quantitatively between patients.

Materials and Methods: We immunohistochemically localized α_1A- and α_1D-adrenoceptor within benign prostatic hyperplasia tissues, and quantitated mRNA expression for these subtypes by real-time quantitative reverse transcription-polymerase chain reaction.

Results: Immunohistochemistry detected both subtypes in stromal but not detected epithelial cells. Copy numbers of α_1A-adrenoceptor mRNA in benign prostatic hyperplasia tissue were significantly higher than those of α_1D-adrenoceptor mRNA. Among patients; the ratio of α_1A- to α_1D-adrenoceptor mRNA ranged from 1.0 to 8.4.

Conclusion: An ideal therapeutic antagonist for treating benign prostatic hyperplasia symptoms should block both α_1A- and α_1D-adrenoceptor

Keywords: Adrenoceptor subtypes; Benign prostatic hyperplasia; Quantitative reverse transcription-polymerase chain reaction

DOI: 10.3126/jpn.v1i1.4439

Journal of Pathology of Nepal (2011) Vol.1, 1-7