Role of Neutrophil CD64 in the Diagnosis of Neonatal Sepsis

Authors

  • Adeel Moideen Department of Nephrology, University of Toronto, Toronto, Ontario, Canada
  • Apurv Barche Department of Paediatrics, Kasturba Medical College Manipal, Manipal Academy of Higher Education (MAHE) Karnataka - 576104, India.
  • Sneha Jaganathan Andrade Department of Paediatrics, Kasturba Medical College Manipal, Manipal Academy of Higher Education (MAHE) Karnataka - 576104, India.
  • Aditya Verma Department of Paediatrics, Kasturba Medical College Manipal, Manipal Academy of Higher Education (MAHE) Karnataka - 576104, India.
  • Leslie Edward Lewis Department of Paediatrics, Kasturba Medical College Manipal, Manipal Academy of Higher Education (MAHE) Karnataka - 576104, India.
  • Jayashree Purkayastha Department of Paediatrics, Kasturba Medical College Manipal, Manipal Academy of Higher Education (MAHE) Karnataka - 576104, India.
  • Ayesha Erum Hadi Department of Radiodiagnosis, McMaster University, Hamilton, Ontario, Canada

DOI:

https://doi.org/10.3126/jnps.v42i2.39821

Keywords:

CD64, hs-CRP, Neonatal sepsi, Neutrophil

Abstract

Introduction: Neutrophil surface CD64 (Cluster of differentiation 64), the highaffinity Fc receptor, is quantitatively up-regulated during infection and sepsis. The diagnostic utility of NCD64 as a reliable marker of neonatal sepsis has not been explored so far. Hence this study has been conducted to compare NCD64 with other currently used infection markers including total leucocyte count, platelet count, absolute neutrophil count (ANC), band:neutrophil ratio and highly sensitive C reactive protein (hs-CRP).

Methods: Consecutively born neonates between March 2014 to November 2014 were enrolled with documented sepsis (n = 81), clinical sepsis (n = 35), and no sepsis (n = 87). NCD64 was analyzed by flow cytometry.

Results: Sepsis episodes had a higher median CD64 index of 10.35 (Range: 15.88, 6.87) as against 2.97 (Range: 5.53, 1.64) in the control group (p < 0.001). The percentage of NCD64 positive cells was also significantly higher in the sepsis group compared to the control group (63.90 ± 2.67 vs 15.07 ± 1.95; p = 0.001). In the ROC curve analysis NCD64, percentage of NCD64 positive cells had the highest AUC (AUC-0.914) using a cutoff of 28.01%, followed by CD64 mean fluorescence intensity (MFI) with an AUC of 0.850 using a cutoff of 5.54. NCD64 was significantly elevated in the groups with documented and clinical sepsis (p < 0.001).

Conclusions: NCD64 is a highly sensitive marker for neonatal sepsis. Prospective studies incorporating NCD64 into a sepsis scoring system are warranted.

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Author Biographies

Apurv Barche, Department of Paediatrics, Kasturba Medical College Manipal, Manipal Academy of Higher Education (MAHE) Karnataka - 576104, India.

Assistant Professor

Sneha Jaganathan Andrade, Department of Paediatrics, Kasturba Medical College Manipal, Manipal Academy of Higher Education (MAHE) Karnataka - 576104, India.

Assistant Professor

Aditya Verma, Department of Paediatrics, Kasturba Medical College Manipal, Manipal Academy of Higher Education (MAHE) Karnataka - 576104, India.

Senior Resident

Leslie Edward Lewis, Department of Paediatrics, Kasturba Medical College Manipal, Manipal Academy of Higher Education (MAHE) Karnataka - 576104, India.

Professor

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Published

2022-12-31

How to Cite

Moideen, A., Barche, A., Andrade, S. J., Verma, A., Lewis, L. E., Purkayastha, J., & Hadi, . A. E. (2022). Role of Neutrophil CD64 in the Diagnosis of Neonatal Sepsis: . Journal of Nepal Paediatric Society, 42(2), 71–76. https://doi.org/10.3126/jnps.v42i2.39821

Issue

Vol. 42 No. 2 (2022)

Section

Original Articles

License

Copyright (c) 2022 Adeel Moideen, Apurv Barche, Sneha Jaganathan Andrade, Aditya Verma, Leslie Edward Lewis, Jayashree Purkayastha, Ayesha Erum Hadi

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