Antimicrobial activities and phytochemical screening of leaf extract of Mikania micrantha H.B.K

  • Anjana Devkota Central Department of Botany, Tribhuwan University, Kirtipur Kathmandu
  • Anita Sahu Central Department of Botany, Tribhuwan University, Kirtipur Kathmandu
Keywords: screening, phytochemical, mycelium, antimicrobial inhibition


Antimicrobial activities and phytochemical screening of leaves of Mikania micrantha was tested in laboratory against phytopathogenic fungi and human pathogenic bacteria. The leaves samples were extracted in distilled water and methanol. The crude extracts of leaves were assessed in-vitro for antimicrobial activity using different concentrations (50, 100, 150,200, and 250 mg/ml) against five fungal strains (viz. Sclerotium rolfsii, Phytopthora capsici, Alternaria brassicae, Fusarium oxysporum and Botrytis cinerea) and six bacterial strains (Klebsiella pneumoniae, Proteus mirabilis, Escherichia coli, Bacillus subtilis, Enterococcusfaecalis and Staphylococcus aureus). Antifungal activity was tested by Poison Food Technique and Linear Mycelium growth Reduction (LMGR) percentage was determined. In crude extractof M. micrantha, the highest LMGR percentage was found in F. oxysporum in both distilledwater and methanol extract. Antibacterial activity was carried out by Disc Diffusion method. In the crude leaf extract of M. micrantha Gram negative bacteria was found more resistant than Gram positive bacteria. Methanol extract was found more effective in determining the Zone of Inhibition for all the strains of bacteria in all the concentrations. The plant extracts were found more effective in showing antibacterial activity than antifungal activity. The phytochemicalscreening revealed that the selected species contained tannin, saponins, alkaloid, flavonoid, cardiac glycosides and terpenoids. This result supports the potential of this plant species used as a new chemotherapeutic drug.


Download data is not yet available.


AIYEGRORO, O A; OKOH A I (2010) Preliminary phytochemical screening and in vitro antioxidant activities of aqueous extract of Helichrysum longifolium. BMC Complementary and Alternative Medicine 10:21–32.

ANTHERDEN, L M (1969) Textbook of pharmaceutical chemistry. Oxford University Press, London, UK (8th edition).

BOHAM, B A; KOCIPAI-ABYAZAN, R (1994) Flavonoids and condensed tannin from leaves of Hawaiian Vccinium vaticulatum and V. calycinium. Pacific scientific 48: 458–463.

CETIN, T E; GURLER, N (1989) Bacterilerin antibiyotiklere duyaarlilik deneyinin yapitnasi. Kukem Dergisi 12: 2–5.

FARNSWORTH, N R (1993) Biological approaches to the screening and evaluation of natural products. In RASOANAIVO, P; RATSIMAMANGA-URVERG, S (eds) Biological evaluation of plants with reference to the Malagasy flora, Madagascar. pp 35–43.

GATSING, D; NKEUGOAUAPI, C F N; NKAH, B F N; KUIATE, J R; TCHOUANGUEP, F M (2010) International Journal of Pharmacology 6: 173–182.

HARBORNE, J B (1973) Phytochemicals methods. Chapman and Hall Ltd., London, UK.

JUST, J; RECIO, M J; GINER, R M; CUÉLLAR, M J; MÁÑEZ, S; BILLIA, A R; RIOS, J L (1998) Antiinflammatory activity of unusual lupine saponins from Bupleurum fruticescens. Planta Medica 64: 404–407.

KHALIL, A; DABABNEH, B F (2007) Inhibition of phytopathogenic fungi by extracts from medicinal plants in Jordan. Journal of Biological Science 7(3): 579–581.

MATAWALI, A; CHIN, L P; ENG, H S; GANSAU, J A (2016) Antibacterial and phytochemical investigations of Mikania micrantha H.B.K. (Asteraceae) from Sabah, Malaysia. Transactions on Science and Technology 3(1–2): 244–250.

MAHIDA, Y; MOHAN, J S (2007) Screening of plants for their potential antibacterial activity Staphylococcus

and Salmonella spp. Natural Product Radiance 6(4): 301–305.

NYARKO, A A; ADDY, M E (1990) Effects of aqueous extract of Adenia cissampeloides on blood pressure and serum analyte of hypertensive patients. Phytotherapy Research 4(1): 25–28.

OKWU, D E (2001) Evaluation of chemical composition of medicinal plants belonging to Euphorbiaceae. Pakistan Veterinary Journal 14: 160–162.

OKWU, D E; OKWU, M E (2004) Chemical composition of Spondias mombin Linn. plant parts. Journal of Environment & Sustainable Agriculture 6(2): 140–147.

OBDONI, B O; OCHUKO, P O (2001) Phytochemical studies and comparative efficacy of the crude extracts of some homostatic plants in Edo and Delta States of Nigeria. Global Journal of Pure and Applied Science 8(b): 203–208.

PANTHI, M P; CHAUDHARY, R P (2006) Antibacterial activity of some selected folklore medicinal plants from West Nepal, Scientific World 4( 4): 16–21.

RUFATTO, L C; GOWER, A ; SCHWAMBACH, J; MOURA, S (2012) Genus Mikania: chemical composition and phytotherapeutical activity. Brazilian Journal of Pharmacognosy 22(6): 1384–1403.

SAMIE, A; NEFEFE, T (2012) Antifungal activities of essential oils from Southern African medicinal plants gainst five Fusarium species. Journal of Medicinal Plants Research 6(3):465–478.

SALAH, N, MILLER, N J ; PAGANGA, G; TIJBURG, L; BOLWELL, G P; RICE- EVANS, C (1995) Polyphenolic flavonoids as scavengers of aqueous phase radicals as chain-breaking antioxidant. Archives of Biochemistry and Biophysics 322:339–346

SODIPO, OA; AKINIYI, JA; OGUNBAMORU, JV (2000) Studies on certain characteristics of extracts of bark of Pansinystalia macruceras (K. Schemp) Pierre Exbeille. Global Journal of Pure and Applied Science 6: 83–87.

SOFOWORA, A (1993) Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd., Ibadan, Nigeria.

STRAY, F (1998) The natural guide to medicinal herbs and plants. Tiger Books International, London, UK.

TAYLOR, J L S; RABE, T; MACGRAWL, L J; JAGER, A K; VAN STADEN, J (2001) Towards the scientific validation of traditional medicinal plants. Plant Growth Regulator 34: 23–27.

TIWARI, S; SIWAKOTI, M; ADHIKARI, B; SUBEDI, K (2005) An inventory and assessment of invasive alien plant species of Nepal. IUCN, The World Conservation Union, Nepal.

TREASE, G E; EVANS, W C (1989) Pharmacognosy. Bailliere Tindall, London, UK (11th edition).

VARMA, J; DUBEY, K (1999) Prospectives of botanical and microbial products as pesticides of tomorrow. Current Science 76: 172–179.

YAO, J; MOELLERING, R (1995) Antibacterial agents. In MURRAY, P; BARON, E; PFALLER, M;

TENOVER, F; YOLKEN, R; ASM, (eds) Manual of Clinical Microbiology. ASM Press; Washington DC, USA; pp 1281–1290.

How to Cite
Devkota, A., & Sahu, A. (2018). Antimicrobial activities and phytochemical screening of leaf extract of Mikania micrantha H.B.K. Journal of Natural History Museum, 30, 274-286.