Effect of sugarcane genotypes and 2, 4-dichlorophenoxy acetic acid on callus induction
DOI:
https://doi.org/10.3126/jiaas.v33i0.20710Keywords:
Sugarcane (Saccharum spp.), Callus, in-vitro, Murashige and Skoog (MS), Endogenous HormoneAbstract
Callus induction is an important step in in-vitro culture valued for micropropagation, somaclonal variation, mutagenesis, synthetic seed production, transformation and genetic engineering. Callusing response of ten sugarcane genotypes viz., BO-110, BO-134, Cose-95422, Cose-98232, Cose-92423, UP-9742, BO-91, BO-135, and Cose-98255, were studied under three different doses (2, 3 & 4 mg L-1) of 2,4-D supplemented in the callus induction media. Callus induction was found to depend on both genotype and 2,4-D concentration in the media. The genotype UP-9742 was most amenable to callus induction (67.50%) followed by Cose-97182 (56.25%) and Cose-92423 (51.00%). While, the media supplemented with 3 mg L-1 2,4-D was found more effective to trigger callus induction in general. Yet, the optimum dose of 2,4-D to be used depends on the interaction of the specific genotype.
Journal of the Institute of Agriculture and Animal Science.
Vol. 33-34, 2015, page: 237-242