Effect of sugarcane genotypes and 2, 4-dichlorophenoxy acetic acid on callus induction

Authors

  • M. Subedi Nepal Agriculture Research Council, Kathmandu
  • B. R. Ojha Agriculture and Forestry University, Chitwan
  • S. K. Ghimire Agriculture and Forestry University, Chitwan
  • B. K. Joshi Nepal Agriculture Research Council, Kathmandu
  • R. K. Niroula Nepal Agriculture Research Council, Kathmandu
  • B. P. Sah Nepal Agriculture Research Council, Kathmandu
  • R. Chaudhary Nepal Agriculture Research Council, Kathmandu
  • R. Kharel Agriculture and Forestry University, Chitwan
  • A. P. Poudel Nepal Agriculture Research Council, Kathmandu

DOI:

https://doi.org/10.3126/jiaas.v33i0.20710

Keywords:

Sugarcane (Saccharum spp.), Callus, in-vitro, Murashige and Skoog (MS), Endogenous Hormone

Abstract

 Callus induction is an important step in in-vitro culture valued for micropropagation, somaclonal variation, mutagenesis, synthetic seed production, transformation and genetic engineering. Callusing response of ten sugarcane genotypes viz., BO-110, BO-134, Cose-95422, Cose-98232, Cose-92423, UP-9742, BO-91, BO-135, and Cose-98255, were studied under three different doses (2, 3 & 4 mg L-1) of 2,4-D supplemented in the callus induction media. Callus induction was found to depend on both genotype and 2,4-D concentration in the media. The genotype UP-9742 was most amenable to callus induction (67.50%) followed by Cose-97182 (56.25%) and Cose-92423 (51.00%). While, the media supplemented with 3 mg L-1 2,4-D was found more effective to trigger callus induction in general. Yet, the optimum dose of 2,4-D to be used depends on the interaction of the specific genotype.

 Journal of the Institute of Agriculture and Animal Science.

Vol. 33-34, 2015, page: 237-242

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Published

2018-08-10

How to Cite

Subedi, M., Ojha, B. R., Ghimire, S. K., Joshi, B. K., Niroula, R. K., Sah, B. P., Chaudhary, R., Kharel, R., & Poudel, A. P. (2018). Effect of sugarcane genotypes and 2, 4-dichlorophenoxy acetic acid on callus induction. Journal of the Institute of Agriculture and Animal Science, 33, 237–242. https://doi.org/10.3126/jiaas.v33i0.20710

Issue

Vol 33-34 (2015)

Section

Research Articles