TY - JOUR AU - Dey, Mohitosh AU - Panda, Sanjib Kumar AU - Sahoo, Lingaraj PY - 2015/12/30 Y2 - 2024/03/29 TI - Establishment of an Efficient Regeneration System Amenable to Agrobacterium Mediated Transformation of Two Elite Indica Rice Varieties of North East India JF - International Journal of Applied Sciences and Biotechnology JA - Int J Appl Sci Biotechnol VL - 3 IS - 4 SE - Research Articles: Biological Sciences DO - 10.3126/ijasbt.v3i4.14005 UR - https://nepjol.info/index.php/IJASBT/article/view/14005 SP - 680-686 AB - <p>An efficient plant regeneration system from embryogenic callus of two elite <em>indica </em>rice (<em>Oryza sativa </em>spp. <em>indica</em>) varieties of Northeast India, Ketokijoha and Monoharsali is established. The effect of auxin, 2,4-dicholorophenoxy acetic acid (2,4-D) on callus induction was optimized. Friable, nodular and creamish-white embryogenic calli were induced from mature seeds on NB medium supplemented with 2.5 mg/l 2,4-D. Plants were regenerated from 40-50 days old embryogenic callus on NB medium containing 0.5 mg/l BAP (6-benzylaminopurine) and 0.25 mg/l ABA (abscisic acid). Regenerated plants with multiple tillers were rooted on half strength MS medium and rooted plants were acclimatized with 94% survival rate. Higher frequency of callus induction as well as plant regeneration was recorded in Ketokijoha as compared to Monoharsali. The calli of both the varieties were found amenable to <em>Agrobacterium-</em>mediated transformation as evident from strong GUS (β-glucuronidase) expression. The results may find wide application for genetic improvement for valuable traits these elite <em>indica </em>rice varieties of Northeast India. </p><p>Int J Appl Sci Biotechnol, Vol 3(4): 680-686</p> ER -