TY - JOUR AU - Gedela, Ravi AU - Makke, Naga Sai Babu AU - Karra, Dinesh PY - 2015/09/25 Y2 - 2024/03/29 TI - A Metagenomics Analysis on B-Carotene Synthesis in Neurospora Crassa JF - International Journal of Applied Sciences and Biotechnology JA - Int J Appl Sci Biotechnol VL - 3 IS - 3 SE - Research Articles: Biological Sciences DO - 10.3126/ijasbt.v3i3.13306 UR - https://nepjol.info/index.php/IJASBT/article/view/13306 SP - 490-503 AB - <p>We have studied <em>insilico</em> on evolutionary uniqueness of phytoene synthase, which is one of the regulatory enzymes of ?-carotene synthesis in <em>Neurospora crassa</em>.  This study reveals multiple sequence alignments showed high sequences with similarity within a species of bacteria, fungi and higher plants.  This results designate interestingly between species of bacteria-fungi, fungi-plant, and among the species of bacteria-fungi-plant, showed tremendously less sequence with similarity, except bacteria-plant (high sequence with similarity) respectively.  In Phylogenetics tree analysis showed within species of bacteria, fungi and plant 91%, 92% and 99% homology.  Whereas in between species of bacteria-fungi, bacteria-plant, fungi-plant, and among the species bacteria-fungi-plant showed 99%, 96%, 100%, and 91%-99% homology respectively.  <em>N. crassa </em>phytoene synthase enzyme encode (Isoprenoid Biosynthesis enzymes, Class 1) protein size 610aa, Cyanobacteria phytoene encode (Isoprenoid Biosynthesis enzymes, Class 1) protein size 310aa, and Oryza sativa Indica phytoene synthase 1 (chloroplast), (Isoprenoid Biosynthesis enzymes, Class 1) encode protein size 421aa (<em>e</em>- value 0.0, 0.0 and 0.0; identity 100%, 100% and 100%; Max.score:1238, 644 and 870) respectively.  We studied <em>insilico</em> on basis of an evolutionary Endosymbiotic theory; a bacterium is the ancestors to eukaryotes. </p><p>Int J Appl Sci Biotechnol, Vol 3(3): 490-503</p> ER -