Assessment of the antibacterial activity of lemongrass-extracted essential oil

Authors

  • Prem Raj Joshi Department of Science and Technology, Kailali Multiple Campus, Far Western University, Nepal
  • Prakash Raj Pant Department of Science and Technology, Kailali Multiple Campus, Far Western University, Nepal
  • Anup Bist Department of Science and Technology, Kailali Multiple Campus, Far Western University, Nepal
  • Gunananda Pant Department of Science and Technology, Kailali Multiple Campus, Far Western University, Nepal
  • Bishweshwar Pant Carbon Composite Energy Nanomaterials Research Center, Woosuk University, Wanju 55338, Republic of Korea
  • Prem Singh Saud Department of Science and Technology, Kailali Multiple Campus, Far Western University, Nepal

DOI:

https://doi.org/10.3126/bibechana.v20i3.58311

Keywords:

Lemongrass, Essential oil, Extraction of essential oil, Anti-bacterial activity

Abstract

Lemongrass is one of the medicinal plants having significant applications to cure various diseases. This study aims at extracting essential oils from lemongrass plants and examining their antibacterial activity. The sample of lemongrass for this study was collected from Kailali District, Nepal. The leaves of lemongrass were collected, washed, cut into small pieces, and dried in the sun. Then pieces of lemongrass were steam distilled for about 4 hours using the Clevenger apparatus, and oil was extracted. The extracted essential oil was further utilized to study its antibacterial activity in human pathogens, namely Staphylococcus aureus (S. aureus: gram-positive bacteria), by Agar diffusion and broth dilution methods. It was found that the zone of inhibition for S. aureus in concentrations of 100%, 75%, 50%, and 25% was about 25 mm, 11 mm, 9 mm, and 0 mm, respectively. In addition, it is clear from this study that the gram-positive bacteria were sensitive to essential oils. The lowest and highest zones of inhibition were shown against S. aureus at 25% and 100% concentrations, respectively. The zone of inhibition at 100% concentration is 25 mm and at 25% concentration is 0 mm, so lemongrass oil is effective against drug-resistant organisms only at high concentrations.

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Author Biographies

Prem Raj Joshi, Department of Science and Technology, Kailali Multiple Campus, Far Western University, Nepal

Assistant Lecturer, Department of Science and Technology 

Prakash Raj Pant, Department of Science and Technology, Kailali Multiple Campus, Far Western University, Nepal

Assistant Professor, Department of Science and Technology 

Anup Bist, Department of Science and Technology, Kailali Multiple Campus, Far Western University, Nepal

Teaching Assistant, Department of Science

Gunananda Pant, Department of Science and Technology, Kailali Multiple Campus, Far Western University, Nepal

Assistant Professor

Bishweshwar Pant , Carbon Composite Energy Nanomaterials Research Center, Woosuk University, Wanju 55338, Republic of Korea

Department of Automotive Engineering, Research Professor 

Prem Singh Saud, Department of Science and Technology, Kailali Multiple Campus, Far Western University, Nepal

Associate Professor, Department of Chemistry 

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Published

2023-11-30

How to Cite

Joshi, P. R., Pant, P. R., Bist, A., Pant, G., Pant , B., & Saud, P. S. (2023). Assessment of the antibacterial activity of lemongrass-extracted essential oil . BIBECHANA, 20(3), 290–296. https://doi.org/10.3126/bibechana.v20i3.58311

Issue

Vol. 20 No. 3 (2023)

Section

Research Articles

License

Copyright (c) 2023 Prem Raj Joshi, Prakash Raj Pant, Anup Bist , Gunananda Pant, Bishweshwar Pant, Prem Singh Saud

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.