In vitro analysis of thimerosal genotoxicity and cytotoxicity effect

Authors

  • Mirela Mačkić-Đurović Associate Professor, Center for Genetics, Faculty of Medicine, University of Sarajevo, Sarajevo, Bosnia and Herzegovina https://orcid.org/0000-0001-7198-5642
  • Dzenana Klepo PhD Candidate, International BURCH University, Sarajevo, Bosnia and Herzegovina
  • Amina Aščerić Postgraduate Resident, Department of Biology, Faculty of Natural Sciences and Mathematics, University of Sarajevo, Sarajevo, Bosnia and Herzegovina https://orcid.org/0000-0002-1512-2858
  • Dunja Rukavina Associate Professor, Department of Biology, Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
  • Izeta Aganović-Mušinović Associate Professor, Department of Immunology, Medical Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina https://orcid.org/0000-0001-5199-3709

Keywords:

Chromosomal aberration; Cytokinesis-block micronucleus cytome assay; Thimerosal

Abstract

Background: Thimerosal is an organomercury compound with high mercury content. Thimerosal is located in the market as an effective bacteriostatic in a series of pharmaceutical products, ophthalmic and nasal products, immunoglobulin preparations, and as a preservative in vaccines.

Aims and Objectives: Since it is a compound with living content, this study aimed to examine the genotoxic and cytotoxic potential of thimerosal in human lymphocytes culture.

Materials and Methods: We used chromosome aberration analysis and cytokinesis-block micronucleus cytome (CBMN-Cyt) assay to test its genotoxic and cytotoxic potential in human lymphocyte culture.

Results: Results showed that the frequency of structural chromosome aberrations and CBMN-cyt assay was significantly increased in treated cultures (1 μg/ml and 0.5 μg/ml) compared to the negative control.

Conclusion: Obtained results and statistical analysis show that thimerosal is genotoxic and cytotoxic in human lymphocytes in tested concentrations.

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Published

2022-03-01 — Updated on 2023-01-25

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How to Cite

Mačkić-Đurović, M., Dzenana Klepo, Aščerić, A., Rukavina, D., & Aganović-Mušinović, I. (2023). In vitro analysis of thimerosal genotoxicity and cytotoxicity effect. Asian Journal of Medical Sciences, 13(3), 53-58. https://doi.org/10.3126/ajms.v13i3.41657 (Original work published 2022)

Issue

Vol. 13 No. 3 (2022)

Section

Original Articles

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Copyright (c) 2022 Asian Journal of Medical Sciences

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How to Cite

Mačkić-Đurović, M., Dzenana Klepo, Aščerić, A., Rukavina, D., & Aganović-Mušinović, I. (2023). In vitro analysis of thimerosal genotoxicity and cytotoxicity effect. Asian Journal of Medical Sciences, 13(3), 53-58. https://doi.org/10.3126/ajms.v13i3.41657 (Original work published 2022)