Correlation of biofilm production with antibiotic susceptibility pattern of Pseudomonas aeruginosa from various clinical specimens

Authors

  • M Swapna Assistant Professor, Department of Microbiology, Vels Medical College and Hospital, Vels University, Manjakaranai, Thiruvallur district, Tamil Nadu, India https://orcid.org/0000-0003-2628-4905
  • G Sumathi Professor and Head, Department of Microbiology, Sri Muthukumaran Medical College Hospital and Research Institute, Dr. M.G.R Medical University, Chikkarayapuram, Chennai, India https://orcid.org/0000-0002-1731-4394
  • M Anitha Lecturer, Department of Microbiology, Shri Sathya Sai Medical College and Research Institute, Sri Balaji Vidyapeeth University, Thiruporur, Tamil Nadu, India https://orcid.org/0000-0003-1440-7218

DOI:

https://doi.org/10.3126/ajms.v13i1.39915

Keywords:

Antibiogram, Biofilm, Microtiter plate method, Pseudomonas aeruginosa

Abstract

Background: Pseudomonas aeruginosa is one of the most prevalent nosocomial pathogens that cause a life-threatening infection. One of the important characteristics of P. aeruginosa is biofilm formation which leads to antibiotic resistance.

Aims and Objectives: The aim of the study was to study the antibiotic resistance pattern of P. aeruginosa isolates and correlation with their biofilm-production.

Materials and Methods: A total of 87 P. aeruginosa isolates from different clinical specimens were processed and confirmed by conventional microbiological methods as per standard methodology. Antibiotic sensitivity testing was done for all isolates. Biofilm producing isolates were identified by the microtiter plate method (MTPM).

Results: Of 87 P. aeruginosa isolates, majority were from pus 33 (38%), followed by urine 26 (30%), sputum 19 (22%), body fluids 7 (8%), and blood 2 (2%). Biofilm producing isolates showed more resistance in comparison to non-biofilm producers. The observed difference between biofilm formation for multidrug resistant and susceptible isolates was found to be statistically significant.

Conclusion: MTPM method was an effective test for detection of biofilm formation and was also able to verify biofilm production by P. aeruginosa. This indicated a higher propensity among the clinical isolates of P. aeruginosa to form biofilm and revealed a positive correlation between biofilm formation and antibiotic resistance. This indicates the need for testing of even susceptible isolates for virulence factors such as biofilm production.

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Published

2022-01-01

How to Cite

Swapna, M., Sumathi, G., & Anitha, M. (2022). Correlation of biofilm production with antibiotic susceptibility pattern of Pseudomonas aeruginosa from various clinical specimens. Asian Journal of Medical Sciences, 13(1), 88–92. https://doi.org/10.3126/ajms.v13i1.39915

Issue

Vol. 13 No. 1 (2022)

Section

Original Articles

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Copyright (c) 2021 Asian Journal of Medical Sciences

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