<i>In-vitro</i> Conservation of <i>Bacopa monniera</i> (L.) Wettst.: A Memory Booster Plant

Authors

  • J. Prabha Department of Botany, University of Rajasthan, Jaipur
  • U. Rani Department of Botany, University of Rajasthan, Jaipur
  • A. Sen Department of Botany, University of Rajasthan, Jaipur
  • R.N. Verma Department of Botany, University of Rajasthan, Jaipur
  • A. Batra Department of Botany, University of Rajasthan, Jaipur

DOI:

https://doi.org/10.3126/on.v8i1.4311

Keywords:

Bacopa monniera (L.) Wettst., in vitro propagation, medicinal herb, growth regulators

Abstract

A mass in-vitro propagation system of Bacopa monniera (L.) Wettst., a traditional medicinal herb, has been developed. Shoot proliferation and growth were greatly influenced by the months of the year during which the explant were collected. High frequency bud break coupled with maximum number of shoots through nodal segment were found in the month of June and August. Of the different growth regulators tried, for in vitro shoot regeneration, MS medium with BAP (1.0 mg/l) induced maximum number of proliferative shoots (65.00±1.453). Multiplication and elongation of the shoots were obtained after regular sub culturing on the same medium after 15-21 days. Rooting from basal end of the shoots occurred on MS medium with the addition of IBA (0.5 mg/l). After a hardening phase of 4 weeks, almost 95% transplantation was success in the field.

DOI: 10.3126/on.v8i1.4311

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How to Cite

Prabha, J., Rani, U., Sen, A., Verma, R., & Batra, A. (2011). <i>In-vitro</i> Conservation of <i>Bacopa monniera</i> (L.) Wettst.: A Memory Booster Plant. Our Nature, 8(1), 40–47. https://doi.org/10.3126/on.v8i1.4311

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