Isolation and Characterization of Three and Four Ring Pahs Degrading Bacteria from Contaminated Sites, Ankleshwar, Gujarat, India

Authors

  • Jignasha G. Patel P.G.Department of Environmental Science and Technology, Institute of Science and Technology for Advanced Studies and Research, Vallabh Vidya Nagar
  • J.I. Nirmal Kumar P.G.Department of Environmental Science and Technology, Institute of Science and Technology for Advanced Studies and Research, Vallabh Vidya Nagar
  • Rita N Kumar Department of Biological and Environmental Sciences, Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidya Nagar- 388 120, Gujarat
  • Shamiyan R. Khan P.G.Department of Environmental Science and Technology, Institute of Science and Technology for Advanced Studies and Research, Vallabh Vidya Nagar

DOI:

https://doi.org/10.3126/ije.v4i1.12184

Keywords:

PAHs contaminated soil, Isolated Bacteria, Biodegradation of PAHs

Abstract

Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria were isolated from prolong contaminated Amalakhadi sediment and crude oil polluted soil Telva, near Ankleshwar Gujarat India. Organisms were treated with two-model PAHs compound Anthracene (ANT), and Pyrene (PYR) as the sole source of carbon and energy. Identification of the isolates was carried out based on their morphological and partial 16S rRNA gene sequences, which revealed that the isolates belong to two main bacterial groups: gram-negative pseudomonas indoxyladons and gram-positive, spore-forming group, Bacillus benzoevorans. GC-MS based degradation study demonstrated that P. indoxyladons efficiently degrade 98% of ANT and PYR by 93.2 % when treated with 250 mg L-1. However, B. benzoevorans could tolerate to 200 mg L-1of PYR. Thus, the findings of the study provide novel bacterial sp. having different capacity to degrade model PAHs compounds and further could be utilized for the standardization of bioremediation protocols for ex situ and in situ studies in aquatic as well as terrestrial ecosystem.

DOI: http://dx.doi.org/10.3126/ije.v4i1.12184

International Journal of Environment

Volume-4, Issue-1, Dec-Feb 2014/15, Page: 130-140

 

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Published

2015-02-22

How to Cite

Patel, J. G., Kumar, J. N., Kumar, R. N., & Khan, S. R. (2015). Isolation and Characterization of Three and Four Ring Pahs Degrading Bacteria from Contaminated Sites, Ankleshwar, Gujarat, India. International Journal of Environment, 4(1), 130–140. https://doi.org/10.3126/ije.v4i1.12184

Issue

Vol. 4 No. 1 (2015)

Section

Research Papers

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