Improvised Media for In Vitro Pollen Germination of some Species of Apocynaceae

Authors

  • Reshmi Chatterjee Birbal Sahni Institute of Palaeobotany, 53 University Raod, Lucknow – 226 007, U.P
  • Satadip Sarkar Department of Botany, Bethune College, Kolkata- 700006, West Bengal
  • GM Narasimha Rao 3Department of Botany, Andhra University, Visakhapatnam-530 003, Andhra Pradesh

DOI:

https://doi.org/10.3126/ije.v3i3.11074

Keywords:

In vitro, Pollen Germination, Apocynaceae, Sitting-Drop Culture, Basic Culture

Abstract

Pollen germination forms one of the most important stage post pollination prior to fertilization. This is essential for proper seed setting and seed development. In vitro pollen germination test is the most reliable way of assessing the pollen viability. In the present study pollen grains of seven genera under Apocynaceae family namely, Allamanda, Alstonia, Catharanthus, Nerium, Plumeria, Thevetia and Tabernaemontana were tested in some basic cultural media, such as Brewbaker’s media, 6% Glucose solution, 4% Calcium Nitrate solution and 3% Boron solution. Alstonia pollen grains exhibited highest percentage of germination rate in all the cultural media. Glucose and Brewbaker’s media is found to be highly suitable for efficient pollen germination in all the genera. Boron solution is effective for germination of pollen grains of tree species. In vitro pollen germination can be easily carried out in laboratories. These results can be utilised in plant breeding programmes to improve cultivar and varieties.

DOI: http://dx.doi.org/10.3126/ije.v3i3.11074

International Journal of Environment Vol.3(3) 2014: 146-153

Downloads

Download data is not yet available.
Abstract
1294
PDF
949

Downloads

Published

2014-09-13

How to Cite

Chatterjee, R., Sarkar, S., & Rao, G. N. (2014). Improvised Media for In Vitro Pollen Germination of some Species of Apocynaceae. International Journal of Environment, 3(3), 146–153. https://doi.org/10.3126/ije.v3i3.11074

Issue

Vol. 3 No. 3 (2014)

Section

Research Papers

License

The author(s) acknowledge that the manuscript submitted is his/her/their own original work; all authors participated in the work in a substantive way and are prepared to take public responsibility for the work; all authors have seen and approved the manuscript as submitted;  the manuscript has not been published and is not being submitted or considered for publication elsewhere; the text, illustrations, and any other materials included in the manuscript do not infringe(plagiarism) upon any existing copyright or other rights of anyone.

Notwithstanding the above, the Contributor(s) or, if applicable the Contributor’s Employer, retain(s) all proprietary rights other than copyright, such as Patent rights; to use, free of charge, all parts of this article for the author’s future works in books, lectures, classroom teaching or oral presentations; the right to reproduce the article for their own purposes provided the copies are not offered for sale.

The copyright to the contribution identified is transferred to IJE.