Role of Urinary Tract Bacterial Infection in the Process of Bladder Carcinogenesis (Molecular and Biochemical Studies)

Authors

  • Abeer Mostafa Ashmawey Cancer Biology Department, National Cancer Institute, Cairo University
  • Waleed S Mohamed Cancer Biology Department, National Cancer Institute, Cairo University
  • Ibrahim M Abdel-Salam Cancer Biology Department, National Cancer Institute, Cairo University
  • Saad M El-Gendy College of Applied Medical Sciences, Qassim University, KSA
  • Ali I Ali Cancer Biology Department, National Cancer Institute, Cairo University
  • Abdelbaset A El-Aaser Cancer Biology Department, National Cancer Institute, Cairo University

DOI:

https://doi.org/10.3126/ajms.v2i1.3542

Keywords:

Bladder carcinogenesis, E. Coli, Soybean, DBA, P16

Abstract

Objective: This work is designed to study the possible role of chronic inflammation induced by E. coli in the urinary bladder of rats, the protective role of soybean flour, in addition to the role of oxidative and nitrosative stresses during bladder carcinogenesis.

Material & Methods: This study was done on one hundred and fifty adult male albino rats (50 - 60 gm) that divided into five groups; a) Normal control group, b) Dibutyl amine and sodium nitrate treated group, c)E.Coli treated group, d) Dibutyl amine and sodium nitrate treated group plus E. coli, and e) Dibutyl amine and sodium nitrate treated group plus soy bean flour. Survival rate and histopatholgical changes during the period of treatment were recorded. Level of malondialdhyde, glutathione, catalase, hydrogen peroxide, Total anti-oxidant capacity and nitric oxide were measured. RNA extracted from bladder tissues was determined in addition to P16 level and caspase-3 expression.

Results: Survival analysis showed a significant decrease (p< 0.001) in E.Coli and dibutyl amine plus sodium nitrate treated groups in comparing with the normal and other treated groups. Also, E. coli infection in the bladder tissues increases the carcinogenic ability of nitrosamine precursors, and enhances oxidative and nitrosative stresses via increasing levels of nitric acid, hydrogen peroxide and malondialdhyde. Regarding the molecular changes, extra bands have been found in E. coli and E. coli + carcinogen treated groups approximately at 16-18KD which are not present in the other groups.

Conclusion: Bacterial infection of the urinary bladder may play a major additive and synergistic role in bladder carcinogenesis. Our results have also shown that soy bean flour may have a protective action during induction of urinary tumors.

Key Words: Bladder carcinogenesis; E. Coli; Soybean; DBA; P16

DOI: 10.3126/ajms.v2i1.3542

Asian Journal of Medical Sciences 2 (2011) 31-40

Downloads

Download data is not yet available.
Abstract
729
PDF
585

Downloads

Published

2011-05-15

How to Cite

Ashmawey, A. M., Mohamed, W. S., Abdel-Salam, I. M., El-Gendy, S. M., Ali, A. I., & El-Aaser, A. A. (2011). Role of Urinary Tract Bacterial Infection in the Process of Bladder Carcinogenesis (Molecular and Biochemical Studies). Asian Journal of Medical Sciences, 2(1), 31–40. https://doi.org/10.3126/ajms.v2i1.3542

Issue

Vol. 2 No. 1 (2011)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. The journal holds copyright and publishes the work under a Creative Commons CC-BY-NC license that permits use, distribution and reprduction in any medium, provided the original work is properly cited and is not used for commercial purposes. The journal should be recognised as the original publisher of this work.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).